Effects of Plasminogen on Sperm-Oocyte Interaction during In Vitro Fertilization in the Pig
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 32권 / 2호
ㆍ저자명 : Soo-JIn Sa, Tae-Shin Kim, Soo-Bong Park, Dong-Seok Lee, Chun-Keun Park
ㆍ저자명 : Soo-JIn Sa, Tae-Shin Kim, Soo-Bong Park, Dong-Seok Lee, Chun-Keun Park
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION ACKNOWLEDGEMENTS REFERENCES영어 초록
Plasminogen activators (PAs) are serine protease that cleave plasminogen to form the active protease plasmin. PA/plasmin system playa role in mammalian fertilization and motility and acrosome reaction of sperm. The present study was undertaken to identify PAs in porcine gametes and investigate a possible role of plasminogen in in vitro fertilization in the pig. When boar spermatozoa were preincubated in a fertilization medium (mTBM) for 0, 2, 4 or 6 h, the activity of tPA-PAI (110~117 kDa), tPA (62~70 kDa), and uPA (34~38 kDa) was observed in the sperm incubation medium and sperm sample. PA activities in the sperm incubation medium significantly (p<0.05) increased according to increasing incubation times, while PA activities in sperm significantly (p<0.05) decreased at the same times. In addition, the rate of acrosome reaction in spermatozoa increased by increasing culture times. When oocytes were separated from porcine cumulus-oocytes complexes at 0, 22 or 44 h of maturation culture, no PA activities were observed in cumulus free-oocyte just after aspiration from follicles. However, the activity of tPA-PAI (108~113 kDa) and tPA (75~83 kDa) was observed at 22 h of in vitro culture and significantly (p<0.05) increased as the duration of the culture increased. On the other hand, when porcine oocytes were activated by sperm penetration or calcium ionophore, plasminogen significantly (p<0.05) increased ZP dissolution time (sec) in activated oocytes by sperm penetration. These results suggest that supplementation of plasminogen to fertilization medium may playa positive role in the improvement of in vitro fertilization ability in the pig.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
Enhanced Green Fluorescent Protein Gene under the Regul..6페이지
Correlations between Sperm Motility, SCSA (Sperm Chroma..7페이지
Analysis of Membrane Integrity, DNA Fragmentation and M..4페이지
Study on the Reproductive Function in Transgenic Pig Ha..5페이지
The Effect of Cryoprotectants on the Survival and In Vi..5페이지