Co-treatment with Demecolcine and BMI-1026, a Potent cdk1 Inhibitor, Induces the Enucleation of Murine Oocytes

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 34권 / 1호
ㆍ저자명 : 최태생

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Collection of Ovulated Oocytes Induction of Oocyte Enucleation and Evaluation Oocyte Fixation and DNA Staining Classification of the Phenotypes of the Oocytes Statistical Analysis RESULTS Classification of the Oocytes into Four Types DISCUSSION REFERENCES

영어 초록

Oocyte enucleation is essential for somatic cell nuclear transfer (SCNT) in the production of cloned animals or embryonic stem cells from adult somatic cells. Most studies of oocyte enucleation have been performed using micromanipulator-based techniques, which are technically demanding, time-consuming, and expensive. Several recent studies have used chemical-induced oocyte enucleation; however, each has been plagued by low efficiency and toxicity. In this study, I found that the co-treatment of murine oocytes with demecolcine and BMI-1026, a potent cdk1 inhibitor, resulted in a high enucleation rate (97%). This method is entirely independent of a micromanipulator and is suitable for the large-scale production of enucleated oocytes. This new method of enucleation will be useful in SCNT and in the development of handmade cloning techniques.

참고 자료

없음