Effects of Reactive Oxygen Species (ROS) on Sperm Function and Plasminogen Activator Activity in Porcine Spermatozoa
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 34권 / 3호
ㆍ저자명 : Soo-Jin Sa, Chun-Keun Park, In-Cheul Kim, Seung-Hoon Lee, Oh-Sub Kwon, Myung-Jick Kim, Kyu-Ho Cho, Dy-Wan Kim, Kyoung-Min So, Hee-Tae Cheong
ㆍ저자명 : Soo-Jin Sa, Chun-Keun Park, In-Cheul Kim, Seung-Hoon Lee, Oh-Sub Kwon, Myung-Jick Kim, Kyu-Ho Cho, Dy-Wan Kim, Kyoung-Min So, Hee-Tae Cheong
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES영어 초록
Plasminogen activators (PAs) are serine protease that cleave plasminogen to form the active protease plasmin and may participate in mammalian fertilization. Although correlations have been reported between reactive oxygen species (ROS) and sperm function, the relationship between PA activity and ROS is unknown. We determined the effects of ROS on sperm function and PA activities in boar spermatozoa preincubated under the X-XO system. When spermatozoa were treated with the X+XO group, a significant increase (p<0.05) was observed in the percentage of acrosome reacted spermatozoa compared with that of the control group. However, when antioxidants were added to the medium with X+XO, the rate of acrosome reaction tended to decrease. Also, a significantly lower percentage of acrosome reacted spermatozoa was observed in the X+XO+catalase group at 6 hr of incubation compared with that of X+XO group. The density of malondialdehyde (MDA) was higher in the X+XO group than in different treatment groups. In another experiment, incubation of spermatozoa in medium with X+XO was associated with a significant (p<0.05) increase in activity of tPA-PAI and tPA compared with the control group. Antioxidants decreased the increased activity of tPA-PAI and tPA by preincubation in the X-XO system. Also, a significantly lower (p<0.05) activities of tPA-PAI and tPA were observed in the X+XO+catalase group compared with the X+XO group. No significant differences, however, were observed in the activity of uPA. These results suggest that the increase of acrosome reaction by the X-XO system resulted in increase of PAs activity in the sperm incubation medium.참고 자료
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