BMP4 처리에 의한 인간 배아줄기세포 유래 KDR 양성 중배엽성 세포군의 분화 양상 조사

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 1호
ㆍ저자명 : 김정모, 손온주, 조윤정, 이재호, 정형민

목차

ABSTRACT 서론 재료 및 방법 인간 배아줄기세포의 배양과 배상체 형성 미분화 인간 배아줄기세포 알칼라인포스파테이즈(AP) 염색 중간엽 세포로의 분화 유도 및 분리방법 중간엽 계통 세포로의 분화 유도 혈관 내피세포의 특성 규명 중간엽 줄기세포의 특성 규명 평활근 세포의 특성 규명 면역형광염색법 결과 미분화 인간 배아줄기세포에서 중배엽성 세포로의 분화유도 미분화 인간 배아줄기세포의 특성규명 BMP4 성장인자 처리에 의해 KDR 발현 양상 변화 KDR/CD133 이중 양성세포의 분리 및 유사 혈관 내피세포로의 분화 KDR/PDGFRα 이중 양성세포의 분리 및 유사 중간엽 줄기세포 및 평활근 세포로의 분화 고찰 인용문헌

영어 초록

The functional cardiovascular system is comprised of distinct mesoderm-derived lineages including endothelial cells, vascular smooth muscle cells and other mesenchymal cells. Recent studies in the human embryonic stem cell differentiation model have provided evidence indicating that these cell lineages are developed from the common progenitors such as hemangioblasts and cardiovascular progenitor cells. Also, the studies have suggested that these progenitors have a common primordial progenitor, which expresses KDR (human Flk-1, also known as VEGFR2, CD309). We demonstrate here that sustained activation of BMP4 (bone morphogenetic protein 4) in hESC line, CHA15 hESC results in KDR+ mesoderm specific differentiation. To determine whether the KDR+ population derived from hESCs enhances potential to differentiate along multipotential mesodermal lineages than undifferentiated hESCs, we analyzed the development of the mesodermal cell types in human embryonic stem cell differentiation cultures. In embryoid body (EB) differentiation culture conditions, we identified an increased expression of KDR+ population from BMP4-stimulated hESC-derived EBs. After induction with additional growth factors, the KDR+ population sorted from hESCs-derived EBs displays mesenchymal, endothelial and vascular smooth muscle potential in matrix-coated monolayer culture systems. The populations plated in monolayer cultures expressed increased levels of related markers and exhibit a stable/homologous phenotype in culture terms. In conclusion, we demonstrate that the KDR+ population is stably isolated from CHA15 hESC-derived EBs using BMP4 and growth factors, and sorted KDR+ population can be utilized to generate multipotential mesodermal progenitors in vitro, which can be further differentiated into cardiovascular specific cells.

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