돼지 체세포복제 배반포에서 Pre-1영역의 Non-CpG 메틸화 양상
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 1호
ㆍ저자명 : 고응규, 임기순, 이휘철, 조상래, 최선호, 최창용, 이풍연, 조창연, 조재현, 유용희
ㆍ저자명 : 고응규, 임기순, 이휘철, 조상래, 최선호, 최창용, 이풍연, 조창연, 조재현, 유용희
목차
ABSTRACT 서론 재료 및 방법 난자의 회수와 체외성숙 Donor Cell의 준비 체세포 복제란의 생산 In Vivo 배반포의 회수 Bisulfite Sequencing에 의한 DNA 메틸화 해석 결과 Pre-1 영역의 Non-CpG 메틸화 Pre-1 영역의 Non-CpG 메틸화 선호도 고찰 인용문헌영어 초록
Previously, we reported that the osmolarity conditions in the satellite region were affected CpG DNA methylation status while Pre-1 sequence was not affected CpG DNA methylation in pNT blastocyst stage. This study was conducted to investigate the DNA methylation status of repeat sequences in pig nuclear transfer (pNT) embryos produced under different osmolarity culture conditions. Control group of pNT embryos was cultured in PZM-3 for six days. Other two treatment groups of pNT embryos were cultured in modified PZM-3 with 138 mM NaCl or 0.05 M sucrose (mPZM-3, 320 mOsmol) for two days, and then cultured in PZM-3 (270 mOsmol) for four days. The DNA methylation status of the Pre-1 sequences in blastocysts was characterized using a bisulfite-sequencing method. Intriguingly, in the present study, we found the unique DNA methylation at several non-CpG sequences at the Pre-1 sequences in all groups. The non-CpG methylation was hypermethylated in all three groups, including in vivo group (86.90% of PZM- 3; 83.87% of NaCl; 84.82% of sucrose; 90.94% of in vivo embryos). To determine whether certain non-CpG methylated sites were preferentially methylated, we also investigated the methylation degree of CpA, CpT and CpC. Excepting in vivo group, preference of methylation was CpT>CpC>CpA in all three groups investigated. These results indicate that DNA methylation of Pre-1 sequences was hypermethylated in CpG as well as non-CpG site, regardless modification of osmolarity in a culture media.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
Establishment of Porcine Embryonic Stem Cells by Aggreg..7페이지
InVitro에서 RetrovirusVectorSystem을 이용한 재조합 hFSH 유전자의 발현7페이지
Nuclear Modeling and Developmental Potential of Bovine ..9페이지
돼지 난자의 체내 및 체외 성숙시 Transducin-like Enhancer Protein 1(T..5페이지
Percoll분리된 미니돼지 정액의 체외 수정 능력에 있어서 동결보존액의 영향7페이지
초자화 동결과 1-단계 융해된 체세포 핵이식란의 직접 이식 기술로 제주흑우 복제소 생산7페이지
Methylation Status of H19 Gene in Embryos Produced by N..9페이지
다양한 Percoll 세척 방법이 동결-융해된 한우 정자의 회수율 및 운동역학에 미치는 영향5페이지
고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향6페이지
EstrogenicXenobiotics가 돼지 정자의 운동성 및 운동역학에 미치는 영향8페이지