Establishment of Porcine Embryonic Stem Cells by Aggregation of Parthenogenetic Embryos
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 1호
ㆍ저자명 : Jin Yu Zhang, Yun Fei Diao, Reza K. Oqani, Je Sok Wo, Dong Il Jin
ㆍ저자명 : Jin Yu Zhang, Yun Fei Diao, Reza K. Oqani, Je Sok Wo, Dong Il Jin
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Chemicals In Vitro Maturation Activation of Porcine Oocytes and In Vitro Culture Aggregation of Embryos and In Vitro Culture Preparation of Feeder Cells and Establishment of EmbryonicStem Cells Real-Time Quantitative Polymerase Chain Reaction Statistical Analysis RESULTS Developmental Ability of Aggregated ParthenogeneticEmbryos Total Cell Numbers in Aggregated and Intact Embryos Analysis of Oct-4 Transcription in Aggregated Blastocysts Establishment of Porcine Embryonic Stem Cells DISCUSSION REFERENCES영어 초록
The pig has been considered to serve as an appropriate model of human disease. Therefore, establishment of porcine embryonic stem cell lines is important. The purpose of the present study was to further work in this direction. We produced porcine parthenogenetic embryos, and separately aggregated two of each of two-cell (2×2), four-cell (2×4), and eight-cell (2×8) embryos derived by parthenogenesis. After culture for 4 days, the developmental ability of the aggregates and total blastocyst cell numbers were evaluated. The percentage of blastocysts was significantly higher in both 2×4- and 2×8-aggregated embryos (58.3±1.9% and 37.2±2.8%, respectively) than in the control or 2×2-aggregated embryos (23.6±1.1% and 12.5±2.4%, respectively). Total blastocyst cell numbers were increased in the 2×4- and 2×8-aggregated embryos (by 44±3.0% and 45±3.3%, respectively) compared with those of control or 2×2-aggregated embryos (30.5±2.1% and 30.7±2.6%, respectively; p<0.05). The levels of mRNA encoding Oct-4 were higher in both the 2×4- and 2×8-aggregated embryos than in the control. When blastocysts derived from 2×4- aggregated embryos or intact normal embryos were cultured on mouse embryonic fibroblast feeder cells to obtain porcine stem cells, blastocysts from aggregated embryos formed colonies that were better in shape compared with those derived from intact blastocysts. Together, the data show that aggregation of porcine embryos not only improves blastocyst quality but also serves as an efficient procedure by which porcine embryonic stem cells can become established.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
InVitro에서 RetrovirusVectorSystem을 이용한 재조합 hFSH 유전자의 발현7페이지
Nuclear Modeling and Developmental Potential of Bovine ..9페이지
돼지 난자의 체내 및 체외 성숙시 Transducin-like Enhancer Protein 1(T..5페이지
돼지 체세포복제 배반포에서 Pre-1영역의 Non-CpG 메틸화 양상5페이지
Percoll분리된 미니돼지 정액의 체외 수정 능력에 있어서 동결보존액의 영향7페이지
초자화 동결과 1-단계 융해된 체세포 핵이식란의 직접 이식 기술로 제주흑우 복제소 생산7페이지
Methylation Status of H19 Gene in Embryos Produced by N..9페이지
다양한 Percoll 세척 방법이 동결-융해된 한우 정자의 회수율 및 운동역학에 미치는 영향5페이지
고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향6페이지
EstrogenicXenobiotics가 돼지 정자의 운동성 및 운동역학에 미치는 영향8페이지