미니돼지 정액의 동결-융해 후 생존율 향상을 위한 동결 조건 확립
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : 이용승, 유한준, 정희태, 양부근, 우제석, 박춘근
ㆍ저자명 : 이용승, 유한준, 정희태, 양부근, 우제석, 박춘근
목차
ABSTRACT 서론 재료 및 방법 정액준비 동결액 준비 및 동결 정액의 동결 속도에 따른 정자의 성상변화 Glycerol 농도와 평형 시간 일반성상 검사 결과 고찰 인용문헌영어 초록
This study was conducted to establish a freezing method of miniature pig spermatozoa. The semen ejaculated from PWG M-type miniature pig was collected by gloved-hand method. The semen was diluted with same volume extender (m-Modena B). The frozen solution used frozen solution of four different (LEY, TCG, BF-5 and m-Modena+egg yolk) for find optimal frozen solution in miniature pig sperm. The diluted semen for frozen rate assay was added to LEY solution (solution Ⅰ: 11% lactose+egg yolk; solution Ⅱ: solutionⅠ+glycerol+OEP), and frozen depending on freezing rate by the three different freezing methods (A: until 5℃ for 1 hrs, holding at —102℃ for 10 min; B: until 5℃ for 2 hrs, holding at —102℃ for 10 min; C: until 5℃ for 3 hrs, holding at —80 and —102℃ for 10 min). Semen cooled until 5℃ was added with glycerol 1, 3 and 5%, and take a equilibrium time for 0, 10 and 30min. Frozen-thawed sperm were evaluated for viability, acrosomal status and morphological abnormality. The results of frozen-thawed sperm ability by frozen solution, viability was higher in LEY solution compared to other three different frozen solution. AR pattern of LEY solution were lower than other three different frozen solution. The results of freezing rate, viability was higher in B method compared to other methods (p<0.05). Acrosomal statute was intacted in A and B methods than C method. The experiment for glycerol condition was showed that sperm viability was higher in extender with 1% and 3% glycerol and equilibrium time of 0 min. The acrosome damage was lower in extender with 1% glycerol and equilibrium time of 10 min than other conditions. In conclusion, the optimal conditions for cryopreservation of miniature pig spermatozoa obtained in LEY frozen solution, cooling rate of 1~2 hrs, 1~3% glycerol concentrations and glycerol equilibrium time of 0~10 min.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
공여세포 처리 조건이 형질전환 복제돼지 생산에 미치는 영향6페이지
수퇘지 정자의 운동성, 생존성 및 체외수정 능력에 대한 시판 액상 정액5페이지
Characterization of X-linked RNA Transcripts in Matured..6페이지
프로게스테론과 에스트라디올 17β에 의한 Long-ChainAcyl-CoA Synthetase4의 ..5페이지
Relationship between In Vitro Maturation and Plasminoge..5페이지
한국의 돼지 인공수정 현황6페이지
200mgFSH투여에 의한 한우의 과배란 유도 및 체내 수정란 생산6페이지
PhosphoproteinEnrichedinAstrocytes15(PEA15)가 과발현하는 형질전환..7페이지
대요크샤 및 랜드레이스종 근교계통돈의 총산자수와 후보유전자에 대한 다형성과 육종가 간의 연관성 분석4페이지
InVitro에서 RetrovirusVectorSystem을 이용한 인간 성장 호르몬 유전자의 발현6페이지