소 정액의 동결 보존시 Methyl-Beta-Cyclodextrin(MBCD)의 영향

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : 이경진, 서기범, 이용승, 유한준, 정희태, 이승환, 양부근, 박춘근

목차

ABSTRACT 서론 재료 및 방법 정액의 준비 정액동결 동결 정액 융해 및 성상 검사 생존율 검사(SYBR-14/PI Staining) 정자 원형질막 기능검사(Hypoosmotic Swelling Test) 첨체 손상율 검사(FITC-PNA/PI Staining) 미토콘드리아 기능율 검사(Rhodamin123/PI Staining) 통계처리 결과 동결 보존액 내 MBCD의 농도별 처리 후 정자의 생존율 비교 평가 정자의 원형질막 기능검사 동결 보존액 내 MBCD의 농도별 처리 후 정자의 첨체 손상검사 정자의 미토콘드리아 기능율 검사 고찰 인용문헌

영어 초록

The objective of this study was to evaluate efficiency of methyl-beta-cyclodextrin (MBCD) in the sperm preservation of bull. For this study, the freezing of diluted semen were added with Triladyl containing 20% egg-yolk and/or 0, 1, 5, 10 and 20 mM MBCD before freezing process. Analysis of viability in frozen-thawed sperm was estimated by SYBR14/PI double stain, hypoosmotic swelling test(HOST) and acrosome damage with FITC-PNA, and mitochondria activation with Rhodamin123 by flow-cytometry. The sperm viability was significantly higher in 0 mM and 5 mM concentrations of MBCD than other groups (p<0.05). However, the HOST was significantly lower at 20 mM concentration of MBCD than other concentrations (p<0.05). In addition, acrosome damage and mitochondria activation rates were significantly lower at 20 mM concentration of MBCD than other groups (p<0.05). In conclusion, the viability of sperm was not significantly different among concentrations of MBCD 0, 5 and 10 mM, but MBCD 20 mM was significantly lower than other groups. In addition, as concentrations of MBCD was high, HOST, acrosome damage and mitochondria activation rates had a negative effect in bull sperm.

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