동물 염색체 분리를 위한 최적 혈액배양 조건에 관한 연구
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : 손시환, 이정업
ㆍ저자명 : 손시환, 이정업
목차
ABSTRACT 서론 재료 및 방법 공시동물 혈액세포 배양 염색체 분리 및 분석 통계분석 결과 고찰 인용문헌영어 초록
In general, the blood cell culture is a common method for animal chromosome preparation. However, every animal and its cells have unique physiological characteristics and functions. Hence, it is very difficult to find the suitable method of chromosome preparation using animal lymphocyte culture. This study was carried out to find the suitable method of chromosome preparation using lymphocytes cultures in mammalians and aves including cattle, rat, mouse and chicken. To seek the optimal method of lymphocyte culture in each animal, 23 factorial experiment was designed. The design evaluated three main effects in culture duration, kinds of mitogen supplements and colcemid exposure time with two levels within each effect. The mitotic index and the score of chromosome morphology were analyzed. In results, the suitable methods of lymphocyte culture for chromosome preparation were 72 hours culture, pokeweed mitogen(PWM) supplement and 90 minutes of colcemid exposure in cattle, 72 hours culture, PWM supplement and 50 minutes of colcemid exposure in chicken, 96 hours culture, concanavalin A supplement and 90 minutes of colcemid exposure in rat, and 72 hours culture, PWM supplement and 50 minutes of colcemid exposure in mouse, respectively. In conclusion, kinds of mitogen, culture duration and colcemid exposure time significantly affected the mitotic index and chromosome morphology in animal lymphocyte culture. The interaction effects between/among treatment factors were also statistically significant.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
공여세포 처리 조건이 형질전환 복제돼지 생산에 미치는 영향6페이지
수퇘지 정자의 운동성, 생존성 및 체외수정 능력에 대한 시판 액상 정액5페이지
Characterization of X-linked RNA Transcripts in Matured..6페이지
프로게스테론과 에스트라디올 17β에 의한 Long-ChainAcyl-CoA Synthetase4의 ..5페이지
Relationship between In Vitro Maturation and Plasminoge..5페이지
한국의 돼지 인공수정 현황6페이지
200mgFSH투여에 의한 한우의 과배란 유도 및 체내 수정란 생산6페이지
PhosphoproteinEnrichedinAstrocytes15(PEA15)가 과발현하는 형질전환..7페이지
대요크샤 및 랜드레이스종 근교계통돈의 총산자수와 후보유전자에 대한 다형성과 육종가 간의 연관성 분석4페이지
InVitro에서 RetrovirusVectorSystem을 이용한 인간 성장 호르몬 유전자의 발현6페이지