Effects of Sodium Nitroprusside and Nitric Oxide on In Vitro Maturation
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : Ji-Hoon Park, Bong-Koo Lee, Young-Ho Chung, Man-Hee Rhee, S. K. Kim
ㆍ저자명 : Ji-Hoon Park, Bong-Koo Lee, Young-Ho Chung, Man-Hee Rhee, S. K. Kim
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Collection of Oocytes In Vitro Maturation of Oocytes Effects of SN and a NO Supplementation Assessment of Meiotic Stage Statistical Analysis RESULTS Effect of SN Concentration on In Vitro Maturation Rate Effect of NO Concentration on In Vitro Maturation Rate Effect of Culture Time and SN or NO on In Vitro Maturation Rate DISCUSSION REFERENCES영어 초록
These study was carried out to investigate the effects of the supplementation with sodium nitroprusside (SN) and nitric oxide (NO) of canine oocytes on IVM rates. Oocytes were incubated in TCM-199 supplement with at 0.03~0.10 mM SN and 0.3~1.0 mM NO for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered mineral oil and cultured in a CO2 incubator (5% CO2, 95% air, 38℃). The in vitro maturation rate of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.03, 0.05, 0.07, 0.10 mM SN were 25.9±3.5%, 36.4±3.2%, 33.3±3.5%, 28.8±3.2%, respectively. The in vitro maturation rate of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.03~0.07 mM SN were significantly increased compare to the control (26.0±2.2%). The in vitro maturation rates of oocytes cultured for 48 hrs in TCM-199 medium supplement with 0.3, 0.5, 0.7, 1.0 mM NO were 28.0±4.2%, 36.5± 3.6%, 30.0±3.8%, 19.2±3.5%, respectively. The in vitro maturation rate of oocytes in TCM-199 medium supplemented with 0.3 and 0.5 mM NO were significantly increased compare to the control (26.0±2.2%). The in vitro maturation rates of oocytes cultured for 12~48 hrs in TCM-199 medium supplement with 0.05 mM SN were 26.0±3.2%, 28.0±3.4%, 38.0±3.2%, respectively. The in vitro maturation rate of oocytes cultured for 12~48 hrs in TCM-199 medium supplement with 0.5 mM NO were 22.0±3.0%, 30.0±3.8%, 36.0±4.2%, respectively. These result was significantly increased compare to the control.참고 자료
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