Effects of Escherichia coli Contamination on Extended Porcine Semen Parameters
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : Kyoung Min So, Soo Jin Sa, Hyo Jin Kim, Ki Hwa Chung, Byeong Yeal Jung, Jung Ho Son, In Cheul Kim
ㆍ저자명 : Kyoung Min So, Soo Jin Sa, Hyo Jin Kim, Ki Hwa Chung, Byeong Yeal Jung, Jung Ho Son, In Cheul Kim
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Animals Semen Preparation Bacterial Isolation Analysis of Sperm Parameters Statistical analysis RESULTS Sperm Motility, Viability and Evaluation of Sperm Agglutination Semen pH DISCUSSION REFERENCES영어 초록
The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, SeminarkPro (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at 17℃ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/Dead® stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at 17℃) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90~6.86) and T4 (6.86~6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0~7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 (20×106 sperm cells/ml : 5×105 cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
GH Increases the Progesterone at Peri-estrus Stage in M..7페이지
Alpha 1,3-Galactosyltransferase Deficiency in Miniature..8페이지
Toxicity and Biomedical Imaging of Fluorescence-Conjuga..8페이지
Effect of MLN8237, a Novel Aurora A Kinase Inhibitor, o..4페이지
Identification and Characterization of Bull Sperm Hyalu..8페이지
Generation of Reactive Oxygen Species and Subsequent DN..5페이지
Effect of Activation of Porcine NT Embryos on In Vitro ..4페이지
Effects of Sodium Nitroprusside and Nitric Oxide on In ..5페이지
Changes in Plasminogen Activity in Uterus Tissue during6페이지
The Effect of Jazf1 Overexpression in Zebrafish Cardiac..5페이지