Identification and Characterization of Bull Sperm Hyaluronidase Hyal5
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 4호
ㆍ저자명 : Ekyune Kim, Kyu-Tae Chang
ㆍ저자명 : Ekyune Kim, Kyu-Tae Chang
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Total RNA Extraction and Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) Southern Blot Analysis Antibody Preparation of Protein Extracts Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis(SDS-PAGE) and Western Blot Analysis Expression of Bovine PH-20 and Hyal5 Zymography Dispersal of Cumulus Cells In Vitro In Vitro Maturation (IVM) and In Vitro Fertilization(IVF) Statistical Analysis RESULTS Expression of PH-20 and Hyal5 Sequence Analysis of Hyal5 and SPAM1 SPAM1 and Hyal5 are Single Copy Genes in the Bovine Genome Importance of Sperm Hyaluronidase in Sperm-Oocyte Interaction DISCUSSION REFERENCES영어 초록
During mammalian fertilization, germ cell-specific hyaluronidases, such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5), are important for the dispersal of the cumulus mass. In this study, we demonstrated that bull Hyal5 is a single copy gene on chromosome 4 that is expressed specifically in the testis. In addition, we expressed recombinant bull SPAM1 and Hyal5 in human embryonic kidney 293T cells and showed that these enzymes possessed hyaluronidase activity. We also demonstrated that a polyclonal antibody against bull sperm hyaluronidase inhibits sperm-egg interactions in an in vitro fertilization (IVF) assay. Our results suggested that bull Hyal5 may have a critical role in bull fertilization.참고 자료
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