The Oxidative Modification of COL6A1 in Membrane Proteins of Ovarian Cancer Patients
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 36권 / 1호
ㆍ저자명 : Hee-Young Yang, Tae-Hoon Lee
ㆍ저자명 : Hee-Young Yang, Tae-Hoon Lee
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Tissue Specimens Two-Dimensional Gel Electrophoresis (2-DE) and WesternBlotting Nano-UPLC MSE Shotgun Proteomics In-gel Tryptic Digestion Nano-UPLC-MSE Tandem Mass Spectrometry Protein and Peptide Identification Bioinformatics Analysis RESULTS Differential Oxidative Modification of Ovarian Cancer Patients Reciprocal Networks of Proteins Containing Cysteine Oxidation Different Modification Pattern of COL6A1 DISCUSSION REFERENCES영어 초록
Ovarian cancer is the most lethal gynecological malignancy, and specific biomarkers are important needed to improve diagnosis, prognosis, and to forecast and monitor treatment efficiency. There are a lot of pathological factors, including reactive oxygen species (ROS), involved in the process of cancer initiation and progression. The oxidative modification of proteins by ROS is implicated in the etiology or progression of disorders and diseases. In this study, a labeling experiment with the thiol-modifying reagent biotinylated iodoacetamide (BIAM) revealed that a variety of proteins were differentially oxidized between normal and tumor tissues of ovarian cancer patients. To identify cysteine oxidation-sensitive proteins in ovarian cancer patients, we performed comparative analysis by nano-UPLC-MSE shotgun proteomics. We found oxidation-sensitive 22 proteins from 41 peptides containing cysteine oxidation. Using Ingenuity program, these proteins identified were established with canonical network related to cytoskeletal network, cellular organization and maintenance, and metabolism. Among oxidation-sensitive proteins, the modification pattern of Collagen alpha-1(VI) chain (COL6A1) was firstly confirmed between normal and tumor tissues of patients by 2-DE western blotting. This result suggested that COL6A1 might have cysteine oxidative modification in tumor tissue of ovarian cancer patients.참고 자료
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