국화재배지의 식물기생선충 분포조사 및 뿌리썩이선충의 ITS와 D3-28S rDNA특성조사
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서지정보
ㆍ발행기관 : 한국응용곤충학회
ㆍ수록지정보 : 한국응용곤충학회지 / 45권 / 3호
ㆍ저자명 : 한혜림, 이재국, 최동로, 한만종, 박병용
ㆍ저자명 : 한혜림, 이재국, 최동로, 한만종, 박병용
영어 초록
A survey was conducted to find out the major plant parasitic nematode in Chrysanthemum morifolium fields in Korea from May to June in 2005. A genus of Pratylenchus was determined as the most important plant parasitic nematode based on analysis of total 50 samples from 8 cities of chrysanthemum field. Pratylenchus showed 86% occurrence rate and average numbered 1,095 per 200㏄ soils and Ig root. Five Pratylenchus isolates, "Muan", "Masan", "Tean", "Gumi", "Jeongup", were selected for the molecular identification of the species of Pratylenchus, and ITS and D3-28S ribosomal DNA were amplified by PCR. For the ITS, only" Muan" isolate was differentiated by total 1 kb PCR amplification, which was 200 bp larger than all the other isolates. There was no size variation in amplified D3-28S rDNA and all isolate represented approximately 320 bp of PCR product. Sequence data of D3-28S rDNA were analysed by MegAlign program in DNASTAR software and phylogenetic tree was constructed. Sequence homology was 100% between "Gumi" isolate and "Tean" isolate and "Jeongup" isolate was also close to these isolates by 99.7% sequence homology. "Gumi", "Tean" group and "Jeongup" isolate were determined to be closely related to Pratylenchus vulnus by 96.7% and 96.3% similarity in respectively. D3 sequence of "Masan" isolate was 100% identical to P. penetrans, and "Muari" isolate showed 99.7% similarity to P. brachyurus. This result was congruent with the branch divergence pattern shown in phylogenetic tree.참고 자료
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