배추좀나방(Plutella xylostella)에 대한 두 곤충병원세균(Xenorhabdus nematophila K1과 Photorhabdus temperata subsp. temperata ANU101) 배양물질의 Bt 병원성 제고 효과

서지정보

ㆍ발행기관 : 한국응용곤충학회 ㆍ수록지정보 : 한국응용곤충학회지 / 48권 / 3호
ㆍ저자명 : 서삼열, 김용균

영어 초록

Two entomopathogenic bacteria, Xenorhabdus nematophila and Photorhabdus temperata subsp. temperata, are known to be potent against the diamondback moth, Plutella xylostella, when the bacteria are injected into the hemocoel. This study investigated any pathogenic effect of their culture broth on P. xylostella by oral administration. Only culture broth of both bacterial species did not give enough pathogenic effects by the oral administration. However, when the culture broth was orally treated together with Bacillus thuringiensis (Bt), both cell-free culture broth significantly enhanced Bt pathogenicity against the 3rd instar larvae of P. xylostella. The culture broth was then fractionated into hexane, ethyl acetate, and aqueous extracts. Most synergistic effect on Bt pathogenicity was found in ethyl acetate extracts of both bacterial species. Thin layer chromatography of these extracts clearly showed that ethyl acetate extracts of both bacterial culture broths possessed metabolites that were different to those of hexane and aqueous extracts. These results suggest that the both entomopathogenic bacteria produce and secrete different factors to give significant synergistic effect on Bt pathogenicity.

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