Progesterone, Estradiol 17 beta 및 Cholesterol Sucrose 층으로부터 정자의 Swim-up 분리에 미치는 영향

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 13권 / 3호
ㆍ저자명 : 김경화, 여영근, 박영식

한국어 초록

난포액에 함유되어 있는 steroids와 sterol이 수정에 참여하는 정자의 주화성에 미치는 영향을 밝히기 위하여, progesterone, estradiol 17 beta 및 cholesterol이 sucrose 층으로부터 정자의 swim-up 분리에 미치는 영향을 조사하였던 바 얻어진 결과는 다음과 같다. 1. Progesterone은 정자의 이동과 운동성을 억제하였으나 수정능획득한 정자를 유인하였으며, 특히 50g/ml 수준의 progestero

영어 초록

This study was carried out to elucidate the effect of progesterone, estradiol 17 beta and cholesterol in follicular fluid on sperm chemotaxis for fertilization. By inducing swim-up migration through sucrose layer into bMSS containing progesterone, estradiol 17 beta and/or cholesterol, their effects on sperm migration and sperm movement were examined. And the results obtained were as follows; 1. Progesterone inhibited sperm migration and movement, but significantly attracted capacitated-sperm at the level of 50 g/ml. 2. Estradiol 17 beta inhibited sperm migration and movement, but didn＇t significantly inhibit migration of capacitated-sperm at the level of 10g/ml. 3. Cholesterol significantly stimulated sperm migration and movement at the level of 50g/ml, but didn＇t attact capacitated-sperm. 4. Progesterone and estradiol 17 beta reduced the effect of cholesterol stimulating sperm migration and movement. But estradiol 17 beta and cholesterol didn＇t reduce the effect of progesterone attracting capacitated-sperm. In conclusion, progesterone of 50g/ml in bMSS attracted the capacitated-sperm, cholesterol of 50g/ml stimulated sperm migration and movement, but estradiol 17 beta of 10g/ml didn＇t affect sperm swim-up separation.

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