난소 수송 온도에 따른 소 체외 수정란의 발육 및 동결-융해 후의 생존성

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 21권 / 2호
ㆍ저자명 : 조상래, 최선호, 김현종, 최창용, 진현주, 손동수

한국어 초록

본 연구는 도축되는 소 난소의 효율적인 이용을 위해서 도축장으로부터 실험실로 운반되는 난소 수송 온도에 따른 체외 수정란 생산 효율을 조사하고자 실시되었다. 도축장의 HACCP 적용으로 도축장 출입이 불가능하므로 위탁하여 난소를 공급받게 되어 취급자의 부주의로 적절한 온도 유지가 되지 않는 경우가 많다. 특히 겨울철에는 더 많은 주의가 필요하다. 따라서 본 실험에서는 겨울철 난소수송 온도에 따라서 4처리 그룹, 즉 (T1), (T2), (T3) 그리고

영어 초록

The present study was carried out to investigate in vitro development and post-thawed survivability of bovine embryos according to different ovary transport temperatures. Bovine ovaries were collected at a local slaughterhouse and were transported at 4 different temperature categories to laboratory: and above (control group). The cumulus-oocyte-complexes aspirated from ovaries were in vitro matured, fertilized and cultured. The rates of maturation (to metaphase II), cleavage and development to blastocysts were compared among treatment groups. Furthermore, frozen-thawed blastocysts were in vitro cultured to compare the survivability among groups. The maturation rates in the T1, T2 and T3 groups () were significantly lower than that in the control group (81.8%, p<0.05). The cleavage rates in the T1 and T2 groups (52.6 and 54.5%) were significantly lower than that in the control group (83.6%, p<0.05). However, there was no difference in the development rate to blastocysts among all groups (, p>0.05). The survivability of frozen-thawed embryos was significantly lower in the T1 group (46.2%) than those in the T2, T3 and control groups (, p<0.05). In conclusion, the results suggest that ovary transport temperature at may be optimal for the better in vitro development and the survival of frozen-thawed embryos produced in vitro Furthermore, exposure of ovary to temperature below during transport may significantly decrease both in vitro development and survivability of frozen-thawed blastocysts.

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