전핵 미세 주입법으로 생산된 한국흑염소 수정란의 이식 조건이 수태율에 미치는 영향
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 22권 / 1호
ㆍ저자명 : 최윤석, 신현국, 장성근, 양홍석, 이옥근, 이두수, 조종기, 신상태
ㆍ저자명 : 최윤석, 신현국, 장성근, 양홍석, 이옥근, 이두수, 조종기, 신상태
한국어 초록
본 연구는 PI한 한국흑염소 수정란의 이식 결과를 통해 수란 흑염소의 수태율에 영향을 미칠 것으로 생각되는 여러 가지 요인을 분석함으로써, 높은 수태율을 얻을 수 있는 수란 흑염소의 최적 조건을 찾아낼 목적으로 수행하였다. 분석 결과, 수태율에 유의적인 영향을 주는 요인들은 발정형태, 수술 빈도, 이식 부위, 황체의 발육 단계, 수정란의 발육단계, 이식된 수정란의 수 등이었다. 자연 발정이 관찰되어 이식된 흑염소(59.1%, 13/22)들이 로 발정이영어 초록
This study was investigated factors affecting the pregnancy rates after transfer of pronuclear microinjected embryos for the production of transgenic Korean black goats. Embryo transfer was carried out in 343 recipient Korean black goats from September 1999 to June 2000. Estrus was induced by the insertion of intravaginal progesterone devices for 2 weeks. A single injection of 400 IU equine chorionic gonadotropin was administered at 48h before removal to increase the proportion of does cycling and ovulation rate. Good quality embryos were prepared by microinjection of DNA into the pronuclei of fertilized goat oocyte and cultured in vitro. Pronuclear microinjected cell stage embryos were surgically transferred into the oviducts of the recipient at day 4 or 5 following removal, and morula to blastocyst stage embryos were surgically transferred into uterus at day 9. Pregnancy was diagnosed by transrectal ultrasound scanning at and 8 weeks following embryo transfer. The pregnancy rate was affected by several factors, such as estrus induction, the number of previous transfer, transfer site, stage of CL (corpus luteum), the number of recipient CL, stage of embryos and the number of transferred embryo. The pregnancy rate was significantly higher in recipients that came into estrus naturally than recipients that induced to come into estrus with (59.1% vs. 36.8%; P<0.05). The pregnancy rate was higher when the embryos were transferred into the left oviduct than transferred into the right oviduct (42.9% vs. 35.3%; P<0.05). The pregnancy rate of recipients with (early) stage corpus hemorrhagicum in ovary was hi틴or than recipient with (late) stage hemorrhagicum (47.5% vs. 17.9%; P<0.01). Higher pregnancy rates were obtained by transfer of 1-cell stage embryos into oviduct while late blastocysts (51.6% vs. 66.7%; P<0.01) into uterus. The pregnancy rates when 3 embryos were transferred to recipients were significantly higher than when 2 embryos we.e transferred (47.6% vs. 27.0%; P<0.05). Although there were no significant difference among the group, adhesion of reproductive organs, uterine size, ovulation rate of recipients, presence of large follicle and difficulty of transfer affected pregnancy rate of recipient. Higher pregnancy rates were obtained in the recipients with diameter uterine horn as compared to the recipients with <5m diameter or >20mm diameter uterine hem (38.9%, 20% vs. 18.2%), in the recipients with large follicle in the ovulated ovary ipsilaterally (53.6% vs. 37.1%) and in the transfer which was carried out easily (39.2% vs. 27.8%, 0%). In conclusion, the high rate of pregnancy was achieved following transfer of pronuclear microinjected embryos when three or four 1-cell stage embryos were transferred into oviduct with stage corpus hemorrhagicum in the ovary of recipient which came into estrus naturally.참고 자료
없음"Journal of Embryo Transfer"의 다른 논문
- 돼지 난자의 체외 수정에 있어서 난구 세포의 존재가 정자 침투율 및 배 발육에 미치는 영향7페이지
- 젖소의 다양한 발정 행동 징후와 배란 시간과의 관계5페이지
- 젖소의 신체충실지수(BCS), 혈장요소태질소(BUN) 수준과 발정 발현과의 관계5페이지
- mSOF 배양액을 이용한 미성숙 난자의 단독 및 그룹 배양 조건을 적용한 일본 흑우 수정란의 성숙,..6페이지
- 소 체외 수정란의 체외 발육에 미치는 Prostaglandins의 영향6페이지
- 비글개에서 발정 휴지기중의 난포낭종5페이지
- PMSG와 hCG 병용 투여에 의한 Shih-tzu 견에서의 인공 발정 유기7페이지
- 더러브렛종 암말의 발정 동기화5페이지
- Sodium Carboxymethylcellulose가 개복 수술한 한국흑염소의 생식기 유착 방지에..5페이지
- 복강경을 이용한 한국흑염소의 성주기 판정6페이지