The Motility of Bovine Sperm and Fertilization Rate of Oocytes during In-Vitro Fertilization following Oviduct Epithelial Cell Co-culture
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국수정란이식학회
ㆍ수록지정보 : Journal of Embryo Transfer / 28권 / 1호
ㆍ저자명 : Hae-Lee Lee, Jae-Hee Park, Yong-Su Kim, Jong Gug Kim
ㆍ저자명 : Hae-Lee Lee, Jae-Hee Park, Yong-Su Kim, Jong Gug Kim
영어 초록
This study examined the motility of either the unattached(upper) or attached(lower) Hanwoo sperm to bovine oviduct epithelial cell(BOEC) monolayers to determine whether there are any changes in their motility during co-culture. The cleavage and blastocyst development rate were compared among different preincubation methods in-vitro, after oocytes were fertilized in-vitro with Hanwoo sperm on BOEC monolayers. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 5 hours and 6 hours (p<0.05) of incubation, in sperm treatment medium without heparin and caffeine. The motility of frozen-thawed sperm in BOEC co-culture group was significantly higher than controls, especially at 3 hours (p<0.05) and 6 hours (p<0.01), in sperm treatment medium containing heparin and caffeine. The motility of the attached( lower) sperm was significantly higher than the unattached(upper) sperm during co-culture with BOEC at all times(p<0.01 or p<0.05), except for 6 hours. After Hanwoo oocytes were fertilized in-vitro with the sperm that had been co-cultured with BOEC in sperm treatment medium containing heparin and caffeine, we determined the cleavage and blastocyst development rate, according to the preincubation methods. Both the cleavage and blastocyst development rate from 2 hour preincubation group were the highest, but significant difference was not recognized. These results show that BOEC plays an important role on sperm hyperactivation related to capacitation regardless of heparin and caffeine in sperm treatment medium. However, oviduct epithelial cell had no significant effect on the development of embryos after in-vitro fertilization in the presence of added heparin and caffeine in sperm treatment medium.참고 자료
없음"Journal of Embryo Transfer"의 다른 논문
Histological Features of the Testis in the Long-beaked ..6페이지
Effects of Ginsenoside-Rg1 on Post-thawed Miniature Pig..9페이지
Analysis of Uterine Bacteria to Increase Reproductive E..7페이지
Microtubule-associated Protein 1B Interacts with Glycer..5페이지
Improvement of Preservation Quality of Chilled Bull Sem..9페이지
Effect of Non Breeding Season on Oocyte Recovery from S..6페이지
Prediction of Parturition Date Based on Vaginal Cytolog..6페이지
Effect of Body Condition Score (BCS) on In Vivo Embryo ..6페이지
Effect of Interferon Supplementation on the Motility of..6페이지
Optimal Derivation Timing for Establishment of Porcine ..6페이지