MicroRNA Analysis during Cultured Odontoblast Differentiation
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 37권 / 3호
ㆍ저자명 : Do Kyung Kim, Min-Gyeong Park, Myoung-Hwa Lee, Sun-Kyoung Yu, Euteum Park, Seog Kim, Seul-Ah Lee, Yeon-Hee Moon, Heung-Joong Kim, Chun Sung Kim
ㆍ저자명 : Do Kyung Kim, Min-Gyeong Park, Myoung-Hwa Lee, Sun-Kyoung Yu, Euteum Park, Seog Kim, Seul-Ah Lee, Yeon-Hee Moon, Heung-Joong Kim, Chun Sung Kim
영어 초록
MicroRNAs (miRNAs, miRs) are about 21-25 nucleotides in length and regulate mRNA translation by base pairing to partially complementary sites, predominantly in the 3’-untranslated region (3’-UTR) of the target mRNA. In this study, the expression profile of miRNAs was compared and analyzed for the establishment of miRNA-related odontoblast differentiation using MDPC-23 cells derived from mouse dental papilla cells. To determine the expression profile of miRNAs during the differentiation of MDPC-23 cells, we employed miRNA microarray analysis, quantitative real-time PCR (qRT-PCR) and Alizaline red-S staining. In the miRNA microarray analysis, 11 miRNAs were found to be up- or down-regulated more than 3-fold between day 0 (control) and day 5 of MDPC-23 cell differentiation among the 1,769 miRNAs examined. In qRT-PCR analysis, the expression levels of two of these molecules, miR-194 and miR-126, were increased and decreased in the control MDPC-23 cells compared with the MDPC-23 cells at day 5 of differentiation, respectively. Importantly, the overexpression of miR-194 significantly accelerated mineralization compared with the control cultures during the differentiation of MDPC-23 cells. These results suggest that the miR-194 augments MDPC-23 cell differentiation, and potently accelerates the mineralization process. Moreover, these in vitro results show that different miRNAs are deregulated during the differentiation of MDPC-23 cells, suggesting the involvement of these genes in the differentiation and mineralization of odontoblasts.참고 자료
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