Effect of Osmolarity of Culture Medium on the Preimplantation Development of Porcine NT and IVF Embryos
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 2호
ㆍ저자명 : In-Sun Hwang, Mi-Rung Park, Hyo-Jin Moon, Joo-Hyun Shim, Dong-Hoon Kim, Byoung-Chul Yang, Yeoung-Gyu Ko, Soh-Suk Yang, Hee-Tae Cheong, Gi-Sun Im
ㆍ저자명 : In-Sun Hwang, Mi-Rung Park, Hyo-Jin Moon, Joo-Hyun Shim, Dong-Hoon Kim, Byoung-Chul Yang, Yeoung-Gyu Ko, Soh-Suk Yang, Hee-Tae Cheong, Gi-Sun Im
목차
ABSTRACT INTRODUCTION MATRRIALS AND METHODS Collection of Oocytes and IVM IVF Preparation of Porcine Fetal Fibroblast Cells Production of NT Embryos In Vitro Culture of Embryos(IVC) Apoptosis Assays Statistical Analysis RESULTS Effects of Sorbitol and Surcrose on Development of IVF Embryos DISCUSSIONS REFERENCES영어 초록
In vitro development of porcine embryo is affected by culture condition. One possible factor is osmolarity of culture medium. This study examined whether high osmolarity of culture medium at the early culture stage improves development of preimplantation porcine in vitro fertilization (IVF) and nuclear transfer (NT) embryos. NT and IVF embryos were divided into three groups and the basic medium was PZM-3 (250~270 mOsmol, control group). The control group of embryos was cultured in PZM-3 for whole culture period. Other two groups of embryos were cultured in a modified PZM-3 with 0.05 M sorbitol or 0.05 M sucrose (300~320 mOsmol, sorbitol or sucrose group) for the first 2 days, and then cultured in PZM-3 for further culture. NT embryos cultured in sucrose group showed a significantly higher developmental rate to the blastocyst stage with a decreased apoptosis rate compared to the sorbitol (p<0.05). For IVF, sucrose group showed a significantly increased the blastocyst formation rate with a decreased apoptosis rate compared to the control (p<0.05). This study represents that the high osmolarity in the early embryo culture stage can enhance the in vitro development of porcine NT and IVF embryos to the blastocyst stage with reduced apoptosis of cells.참고 자료
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