The mRNA Expression and Methylation Pattern of Apoptosis-related and Imprinted Genes in Day 35 of Cloned Pig Fetuses
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 4호
ㆍ저자명 : Hyun-Ju Jung, Yeoung-Gyu Ko, Seongsoo Hwang, Gi-Sun Im, Mi-Rung Park, Jae-Seok Woo, Choon-Keum Park, Hwan-Hoo Seong
ㆍ저자명 : Hyun-Ju Jung, Yeoung-Gyu Ko, Seongsoo Hwang, Gi-Sun Im, Mi-Rung Park, Jae-Seok Woo, Choon-Keum Park, Hwan-Hoo Seong
목차
ABSTRACT IVTRODUCTION MATERIALS AND METHODS Recovery of Control or Cloned Fetuses Total RNA Extraction and cDNA Synthesis Quantitative Real-Time PCR Preparation of Genomic DNA Sodium bisulfite Genomic Sequencing Statistical Analysis RESULTS Recovery Data of Fetuses from Uterus mRNA Expression of Apoptosis-Related and Imprinted Genes DMR Methlation Pattern in H19 Gene DISCUSSION REFFTENCES영어 초록
This study was conducted to examine the mRNA expression of apoptosis-related and imprinted genes and methylation pattern of the differentially methylated region (DMR) of H19 gene in day 35 of SCNT pig fetuses. The day 35 of natural mating (control) or cloned (clone) pig fetuses were recovered from uterus. Endometrium from dam and liver from fetus were obtained, respectively. mRNA expression was evaluated by real-time PCR and methylation pattern was analyzed by bisulfite sequencing method. The Bcl-2 mRNA expression in clone was significantly lower than that of control (p<0.05). The mRNA expression of H19 gene in both endometrium and liver was significantly higher in clone than that of control, respectively (p<0.05). The level of IGF-2 mRNA in liver of clone was significantly lower than that of control (p<0.05), whereas the mRNA expression of IGF2-R gene in liver of clone was significantly higher than that of control (p<0.05). The DMR of H19 was lower methylation pattern in clone than that of control. These results suggest that the aberrant mRNA expression of apoptosis-related and imprinted genes and the lower DMR methylation pattern of imprinted gene may be closely related to the inadequate fetal development of cloned fetus.참고 자료
없음"Reproductive & developmental biology"의 다른 논문
Differential Expression of TPX2 upon Differentiation of..6페이지
Viability of Somatic Cell Nuclear Transfer Embryos foll..6페이지
Supression of DNMTs Accelerates the In Vitro Erythropoi..8페이지
The Effect of Prostaglandin F2α on Semen Collection Tra..4페이지
Comparative Study of Protein Profile during Development..8페이지