Molecular Cloning of Vps26a, Vps26b, Vps29, and Vps2635 and Expression Analysis of Retromer Complex in Micro Pig

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 32권 / 1호
ㆍ저자명 : Ekyune Kim, Young-Hyun Kim, Chun-Hun Ryu, Jae-Woong Lee, Sang-Hyun Kim, Sang-Rae Lee, Myeong-Su Kim, Wanb-Jun Kim, Jeong mook Lim, Kyu-Tac Chang

목차

ABSTRACT INTRODUCTION MATERIALS AND METHODS Animal Total RNA Extraction and cDNA Cloning of Vps26a, Vps26b, Vps29, and Vps35 Genes from Micro Pigs Preparation of Protein Extracts Western Blot Analysis Recombinant DNA Constructs Yeast Two-Hybrid Assay RESULTS Isolation and Characterization of the Retromer Components Vps26a, Vps29, and Vps35 from Micro Pigs Retromer Complex Formations among Vps26a, Vps26b, Vps29, and Vps35 Micro Pig Vps35 Interacts with Micro Pig Vps26a, Vps26b, and Vps29 in Yeast Two-Hybrid System DISCUSSION REFERENCES

영어 초록

Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

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