Lentivirus-mediated Gene Transfer to Bovine Embryos
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 32권 / 1호
ㆍ저자명 : Young Mi Kim, Mo Sun Kwon, Bon Chul Koo, Teoan Kim, Heng-Cherl Yom, Dae Hwan Ko
ㆍ저자명 : Young Mi Kim, Mo Sun Kwon, Bon Chul Koo, Teoan Kim, Heng-Cherl Yom, Dae Hwan Ko
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Nomenclature Construction of Retrovirus vectors Virus Production and Infection In Vitro Maturation (IVM) Microinjection of Retroviral Vectior into Perivitelline Space In Vivo Fertilization (IVF) of Oocytes RT-PCR Analysis Westem Analysis RESULTS Expression of EGFP in Bovine Fetal Fibroblasts Infceted with either FLV-CGW of HLV-OGW Recombinant Virus Expression of EGFP in Bovine Embryos Transduced with either FLV-CGW or HLV-CGW Recombinant Virus DISCUSSION REFERENCES영어 초록
Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.참고 자료
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