Development of Cryo-banking System of Lilium Species
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서지정보
ㆍ발행기관 : 한국화훼학회
ㆍ수록지정보 : 화훼연구 / 22권 / 3호
ㆍ저자명 : Jung Yoon Yi, Gi An Lee, Sok Young Lee, Jong Wook Chung
ㆍ저자명 : Jung Yoon Yi, Gi An Lee, Sok Young Lee, Jong Wook Chung
목차
AbstractIntroduction
Materials and Methods
Planting material
Culturing of plant material
Cold-hardening and pre-culture
Loading and dehydration process
Thawing, unloading and plant regeneration
Morphological analysis
Results and Discussion
Conclusions
Acknowledgments
References
영어 초록
Germplasm collection of lily in the mountainousarea is laborious and time consuming accompanied withhigh costs and high risk due to inappropriate environmentalconditions. Cryopreservation being an ideal method for thelong-term preservation can be employed in conservation ofvaluable lily germplasm. Previously, we developed cryo-preservation protocol for Lilium germplasm using ‘dropletvitrification’. In this paper, we have chosen shoot tips as amaterial for cryopreservation because of their genetic safetyupon regrowth in tissue culture. Using this protocol, wehave preserved approximately 160 accessions of lily germ-plasm in 2010~2012. The regeneration rates are rangedfrom 54.3% to 58.5% while the survival rates were from58.3% to 66.4%. Among Lilium germplasm cryopreserved,there are some Korean, Chinese, and Taiwanese seedstocks which have good qualities for inter-species hybrid.Moreover, we also conserved Korean wild endangered seedstock, especially Lilium hansonii. The morphological studyof Lilium germplasm regenerated from cryopreserved mate-rial confirmed the stability of clonal material following cryo-preservation. We anticipate this cryo-collection will beavailable and useful to curators or breeders of Lilium andthis cryobank will also facilitate the conservation and inter-national exchange of Lilium germplasm.참고 자료
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