Induction of Anticarcinogenic Enzymes by Dichloromethane-soluble Fraction of Physalis alkekengi var. francheti Hort. in Mouse Hepatoma Cells
(주)코리아스칼라
- 최초 등록일
- 2016.04.02
- 최종 저작일
- 2014.09
- 6페이지/ 어도비 PDF
- 가격 4,000원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 경북대학교 농업생명과학대학
ㆍ수록지정보 : Current Research on Agriculture and Life Sciences / 32권 / 3호
ㆍ저자명 : Seo, JiYeon, Kim, Hyo Jung, Kim, Jong-Sang
목차
Abstract
Introduction
Materials and Methods
Materials
Cell culture
Preparation of samples
MTT cytotoxicity assay
Quinone reductase activity assay
Assay of reporter gene activity
Western blot analysis
Statistical analysis
Results
Extraction rate of sample according to experimental setting
Anti-proliferative effects of extract and solvent fractions from P. alkekengi var. francheti Hort. in mouse hepatoma cells.
Induction levels of QR using extract and solvent fractions of P. alkekengi var. francheti Hort. in mouse hepatoma cells.
Effect of extract and solvent fractions of P. alkekengi var. francheti Hort. on pARE-TI-Luciferase expression
Effect of extract and solvent fractions of P. alkekengi var. francheti Hort. on phase 2 enzyme expression in hepa1c1c7 cells
Discussion
Acknowledgments
References
영어 초록
Physalis alkekengi var. francheti Hort. is known as an insecticide and traditional remedy for liver related diseases. Therefore, this study investigated the chemopreventive effects of extracts and several solvent fractions (n-hexane, dichloromethane, n-butanol, water) of Physalis alkekengi var. francheti Hort. First, their cytotoxicity and NQO1 activity were measured using an MTT assay, plus a quinone reductase [NAD(P)H dehydrogenase (quinone); NAD(P)H: (quinone acceptor) oxidoreductase, EC 1.6.99.2]-inducing activity assay was performed using cultured murine hepatoma cells (Hepa1c1c7) and its mutant cells(BpRc1). The reduction of electrophilic quinones by NQO1 is an important detoxification pathway and major mechanism of chemoprevention. When compared with the other solvent soluble fractions with different polarities, the dichloromethane fraction of Physalis alkekengi var. francheti Hort. showed a higher NQO1-inducing activity that was also dose-dependent. Moreover, the dichloromethane fraction of Physalis alkekengi var. francheti Hort. induced ARE-luciferase activities in HepG2-C8 cells that were generated by transfecting the ARE-luciferase gene construct, suggesting the Nrf2-ARE-mediated induction of anti-oxidative enzymes. In conclusion, the dichloromethane-soluble fraction of Physalis alkekengi var. francheti Hort. showed a relatively strong induction of detoxifying enzymes, thereby meriting further study to identify the active components and evaluate their potential as cancer preventive agents.
참고 자료
없음
"Current Research on Agriculture and Life Sciences"의 다른 논문
더보기 (4/9)