Effect of probiotic 379D as an alternative to antibiotics for feed supplementation in broiler chickens

서지정보

ㆍ발행기관 : 충북대학교 동물의학연구소 ㆍ수록지정보 : Journal of Biomedical Research / 16권 / 1호
ㆍ저자명 : Se Eun Kim, Kyung Mi Shim, Jong Choon Kim, Yun Hee Choi, Jin Cheol Yoo, Seong Soo Kang

목차

Introduction
Materials and Methods
Test substance
Preparation of crude antimicrobial peptide
In-gel activity assay for detecting bacteriocin activity
Antimicrobial susceptibility test
Experimental birds and management
Experimental groups
Clinical observations and mortality survey
Body weights and feed consumption measurements
Statistical Analysis
Results
Characterization of the antimicrobial peptide
Inhibition spectrum and sensitivity of the antimicrobial peptide
Clinical signs and mortality
Body weight changes, feed consumption, and feed efficiency
Discussion
Acknowledgements
ORCID
References

영어 초록

Antibiotics have been used to prevent disease, promote growth rate, and improve feed efficiency. However, the use of antibiotics in livestock has been restricted worldwide due to problems such as bacterial resistance. Therefore, probiotics among alternatives to antibiotics have gained attention in the livestock feed industry these days. This study was conducted to investigate the effects of dietary supplementation with probiotic 379D on safety, growth rate, and feed efficiency. In this study, bacterial strain 379D was isolated from soil and identified as a Bacillus sp. according to 16S rRNA sequence analysis. In an in vitro test, in-gel activity assay and antimicrobial susceptibility test were conducted to evaluate 379D. In an in vivo study, 379D was administered at concentrations of 0.1% and 1% to broiler chickens for 28 days. The results of in-gel activity assay and antimicrobial susceptibility test showed that strain 379D had broad spectrum antimicrobial activity. Furthermore, no adverse 379D-related effects were observed in 0.1% and 1% groups. Feed efficiency was higher in the 379D-treated groups than in the control group. In conclusion, 379D is expected to be used as a safe alternative to antibiotics in a feed supplement and will improve feed efficiency in broiler chickens.

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