Stimulation of keratinocyte differentiation by extract of combined medicinal plant

서지정보

ㆍ발행기관 : 충북대학교 동물의학연구소 ㆍ수록지정보 : Journal of Biomedical Research / 15권 / 4호
ㆍ저자명 : Xue Mei Li, Dae-Kyoung Choi, Seul Ki Lim, In Suk Ahn, Dong-Il Kim, Yong Jun Piao, Chang Deok Kim, Jeung-Hoon Lee

목차

Introduction
Materials and Methods
Cell culture
Extract of combined medicinal plants (ECMP)
MTT assay
Western blot analysis
Luciferase reporter assay
Cell growth analysis
Transepidermal water loss (TEWL)
Immunohistochemistry
Statistical analysis
Results
Cytotoxicity of ECMP
Effect of ECMP on keratinocyte differentiation
Effect of ECMP on keratinocyte proliferation
Effect of ECMP on skin barrier functionality
Discussion
Acknowledgements
ORCID
References

영어 초록

Skin is the outermost organ and acts as a barrier between the organism and environment. Skin protects the organism from environmental insults, such as chemicals, pathogens, and UV light. Much of the protective function of skin is dependent on the epidermis, a multi-layered epithelium that is composed of various cell types such as keratinocytes and melanocytes. Keratinocytes produce protective components through a sophisticated differentiation process. Disturbance of keratinocyte differentiation is related to several skin diseases such as psoriasis and atopic dermatitis. In this study, we prepared extract of combined medicinal plants (ECMP) consisting of Taraxacum platycarpum H. Dahlstedt, Heartleaf Houttuynia, Glycyrrhiza uralensis Fischer, and root bark of Ulmus davidiana. We demonstrated that ECMP enhanced keratinocyte differentiation and barrier functionality using an in vitro cell culture system and in vivo animal test. Treatment of cultured keratinocytes with ECMP resulted in induction of keratinocyte differentiation, as evidenced by increased differentiation markers such as involucrin, loricrin, and filaggrin. In line with these results, ECMP decreased proliferation of keratinocytes cultured in vitro. ECMP applied topically to tape-stripped mouse skins accelerated reduction of transepidermal water loss (TEWL), indicating fast recovery of barrier function. Immunohistochemistry showed that ECMP increased the filaggrin level in tape-stripped mouse skins. These results suggest that ECMP may be applicable for keratinocyte differentiation-related skin diseases.

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