구강암의 광역학 치료 모델로서 삼차원 배양법의 효용성

서지정보

ㆍ발행기관 : 대한구강악안면병리학회 ㆍ수록지정보 : 대한구강악안면병리학회지 / 34권 / 2호
ㆍ저자명 : 백종필, 안상건, 윤정훈

목차

I. 서론
II. 연구재료 및 방법
1. 구강암 세포의 삼차원 배양
2. 삼차원 배양 구강암의 광역학 치료
3. 병리조직, 면역조직화학 및 TUNEL 분석
4. 통계 분석
III. 연구결과
1. 삼차원 배양(oraganotypic raft culture) 구강암조직
2. 삼차원 배양 구강암의 광역학 치료 후 병리학적 소견
3. 삼차원 배양 구강암의 광역학 치료후 PCNA 발현의면역조직화학적 평가
4. 삼차원 배양 구강암의 광역학 치료후 TUNELassay 평가
IV. 고찰
V. 참고문헌

영어 초록

The organotypic raft culture model has been shown to be a useful method for examining the effects of biochemical manipulation on various epithelial cell types, using in vitro conditions that simulate the in vivo environment of the tissue of origin. To investigate this method as a model for photodynamic therapy (PDT), we cultures the oral squamous carcinoma cell line YD-10B on fibroblast-containing collagen gels at the air-liquid interface and assessed the efficacy of PDT using a photosensitiser 5-ALA-hexenyl ester. PDT effect on YD-10B organotypic raft cultures after twenty- four hours was determined. The number of residual cells was significantly reduced in comparison to control at all four different conditions. PCNA immunostaining and TUNEL assay revealed that PDT inhibited cell proliferation and increased apoptosis. These findings suggest that the organotypic raft culture model provides an effective and rapid laboratory method of investigating strategies for PDT on oral precancerous lesions and squamous cell carcinomas

참고 자료

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