단삼표준화시료 PF2401-SF의 사염화탄소에 의한 간독성 보호기전

서지정보

ㆍ발행기관 : 대한구강악안면병리학회 ㆍ수록지정보 : 대한구강악안면병리학회지 / 36권 / 5호
ㆍ저자명 : 김웅현, 손동환, 이성희

목차

Ⅰ. INTRODUCTION
II. MATERIALS and METHODS
1. Chemicals and Reagents
2. Preparation of PF2401-SF
3. Animals and treatment
4. Western blot analysis
5. Statistical analysis
III. RESULTS
1. Protective effects of PF2401-SF on the liver of mice
2. Effect of PF2401-SF on hepatic HO-1 expression
3. Effect of PF2401-SF on JNK phosphorylation
IV. DISCUSSION
VI. REFERENCES

영어 초록

We previously prepared a standardized and purified extract of Salvia miltiorrhiza, PF2401-SF, and showed that it protected against hepatic injury more effectively than ethanol based extraction. In this study, we determined the hepatoprotective mechanisms of PF2401-SF in vivo. Hepatic injury was induced in mice by using carbon tetrachloride (CCl4). Treatment with PF2401-SF (1 or 10 mg/kg, p.o.) significantly reduced the levels of alanine transaminase (ALT) and aspartate transaminase (AST) in the plasma. PF2401-SF treatment resulted in further elevation of the CCl4-induced heme oxygenase-1 (HO-1) expression, which contributed to the PF2401-SF-mediated liver protection. Additionally, PF2401-SF treatment significantly reduced the c-Jun NH2-terminal kinase (JNK) phosphorylation induced by CCl4. Taken together, these results suggest that the protective effect of PF2401-SF, a standardized fraction of S. miltiorrhiza, against CCl4-induced hepatic injury in mice arises from its induction effect on HO-1 and inhibitory effect on JNK phosphorylation.

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