Factors Influencing the Efficiency of In Vitro Embryo Production in the Pig
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- 2016.04.02
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 39권 / 2호
ㆍ저자명 : Tao Lin, Jae Eun Lee, Hyun Young Shin, Reza K. Oqani, Dong Il Jin
목차
ABSTRACT
INTRODUCTION
FOLLICLE DIAMETER
COC MORPHOLOGY
OOCYTE RECOVERY METHOD
IVM OF OOCYTES
DENUDING PROCEDURES
MORPHOLOGY OF THE FIRST POLAR BODY
EMBRYO CULTURE MEDIUM ANDCONDITIONS
RELATIONSHIP BETWEEN EMBRYOQUALITY AND CULTURE TIME
IN VITRO FERTILIZATION
PARTHENOGENETIC ACTIVATION
SOMATIC CELL NUCLEAR TRANSFER
IN SUMMARY
REFERENCES
영어 초록
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.
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