Effects of Different Sucrose Concentrations in the Culture Medium on the Growth of Colored Zantedeschia In Vitro under CO2 Enrichment Conditions
(주)코리아스칼라
- 최초 등록일
- 2016.09.03
- 최종 저작일
- 2016.06
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서지정보
ㆍ발행기관 : 한국화훼학회
ㆍ수록지정보 : 화훼연구 / 24권 / 2호
ㆍ저자명 : Zheng Wang, Yinglong Song, Liyun Shi, Yuzhen Guo, Wenqian Shang, Dan He, Song Lin He
목차
Introduction
Materials and Methods
Materials, treatments and culture conditions
Measurement, calculation and statistical analysis
Results
External morphological characteristics
Fresh weight, dry weight and dry mass rateof plantlets in vitro
Expression patterns of leaf chlorophyll content(SPAD value)
Root vigor of plantlets in vitro under CO2Enrichment
Discussion
References
영어 초록
Carbon source, an essential nutrient for plant growth, mainly includes exogenous sugar and CO2 of the environment in vitro. Therefore, the exogenous sugar and CO2 of the environment make the important roles in tissue culture. The aim of this study is to investigate the effects of different sugar concentrations (0, 10, 15 and 30 g·L-1) on the growth of colored Zantedeschia in vitro under certain CO2 concentration and explore the optimal sugar concentration. The plantlets in vitro of colored Zantedeschia had the largest root number, root weight, and root vigor under 0 g·L-1 (sugar-free culture) treatment. And they had the largest plant height, leaf length and leaf chlorophyll content, but p oor r oot v igor under 3 0 g·L-1 sugar. This study indicated that the optimal condition for proliferation and seedling culture of colored Zantedeschia plantlets in vitro was MS medium with 30 g·L-1 sugar, and the suitable medium for rooting culture and transplanting of colored Zantedeschia was MS medium with sugar-free culture under CO2 enrichment condition.
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