In Vitro Antioxidant Properties of Equisetum arvense and Its Effects on Serum Lipid Levels in Mice Fed a High-Fat Diet
(주)코리아스칼라
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- 2016.09.03
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- 2016.06
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서지정보
ㆍ발행기관 : 한국식품영양학회
ㆍ수록지정보 : 한국식품영양학회지 / 29권 / 3호
ㆍ저자명 : Yong-Hwan Kim, Kyung-Ok Shin, Kyung-Soon Choi
목차
Introduction
Materials and Methods
1. Plant materials and extraction
2. Preparation of samples for antioxidant analysis
3. Determination of total phenolic content (TPC)
4. ABTS radical scavenging assay
5. DPPH radical scavenging assay
6. Reducing power assay
7. Polyphenol compound analysis by HPLC
8. Animal experiments
9. Analysis of blood lipids
10. Statistical analysis
Results
1. Total phenolic content (TPC)
2. ABTS radical scavenging activity
3. DPPH radical scavenging activity
4. Reducing power activity
5. Phenolic contents by HPLC
6. Animal experiments
Discussion
References
영어 초록
This study analyzed the antioxidant properties of Equisetum arvense and its effects on serum factor levels in mice fed a high-fat diet. The aim was to establish a new effective resource for biologically active materials. E. arvense stem and root extracts were obtained using deionized water at 95℃, and 70.5% ethanol at 85℃. These extracts were used to analyze the total phenolic compounds and antioxidant (ABTS, DDPH, and FRAP) activities. The effects of prepared ground samples were evaluated by feeding them to mice. E. arvense extracts showed strong antioxidant effects. The caffeic acid content was highest in the 70.5% ethanol extract of the vegetative stem, as determined by high-performance liquid chromatography. The blood concentrations of insulin and leptin were significantly lower in mice fed a high-fat diet supplemented with extracts of the root, reproductive stem, and vegetative stem of E. arvense than in mice fed only a high-fat diet. These results suggest that the polyphenolic compounds in E. arvense extracts exert various antioxidant effects. The stems and root of E. arvense can lower the blood levels of insulin and leptin, even after consumption of a high-fat diet.
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