Inhibition of biofilm formation of periodontal pathogens by D-Arabinose
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 46권 / 3호
ㆍ저자명 : Sun-Jin An, Jong-Uk Namkung, Kyung-Won Ha, Hye-Kyoung Jun, Hyun Young Kim, Bong-Kyu Choi
ㆍ저자명 : Sun-Jin An, Jong-Uk Namkung, Kyung-Won Ha, Hye-Kyoung Jun, Hyun Young Kim, Bong-Kyu Choi
목차
IntroductionMaterials and Methods
1. Bacterial culture
2. Effect of D-Arabinose on growth of planktonicbacteria
3. Biofilm formation assay
4. Effect of D-Arabinose on F. nucleatum AI-2 activity
5. Quantitative polymerase chain reaction (qPCR)
6. Sodium dodecyl-sulfate polyacrylamide gelelectrophoresis (SDS-PAGE) of D-Arabinosetreatedbacteria
7. Statistical analysis
Results
1. Inhibitory effect of D-Arabinose on biofilmformation of periodontal pathogens
2. Inhibition of F. nucleatum AI-2 activity byD-Arabinose
3. Inhibition of expression of virulence factors byD-Arabinose
4. Modification of protein profiles of periodontalpathogens by D-Arabinose
Discussion
References
영어 초록
Periodontitis and periimplantitis are caused as a result of dental biofilm formation. This biofilm is composed of multiple species of pathogens. Therefore, controlling biofilm formation is critical for disease prevention. To inhibit biofilm formation, sugars can be used to interrupt lectin-involving interactions between bacteria or between bacteria and a host. In this study, we evaluated the effect of D-Arabinose on biofilm formation of putative periodontal pathogens as well as the quorum sensing activity and whole protein profiles of the pathogens. Crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy revealed that D-Arabinose inhibited biofilm formation of Porphyromonas gingivalis , Fusobacterium nucleatum , and Tannerella forsythia . D-Arabinose also significantly inhibited the activity of autoinducer 2 of F. nucleatum and the expression of representative bacterial virulence genes. Furthermore, D-Arabinose treatment altered the expression of some bacterial proteins. These results demonstrate that D-Arabinose can be used as an antibiofilm agent for the prevention of periodontal infections.참고 자료
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