Inhibitory effects of Coptis chinensis extract on the growth and biofilm formation of Streptococcus mutans and Streptococcus sobrinus
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 45권 / 4호
ㆍ저자명 : Si Yeong Kim, Yuri Song, Hyun Ah Lee, Hee Sam Na, Chul Jong Jung, Gyung Yun Bek, and Jin Chung
ㆍ저자명 : Si Yeong Kim, Yuri Song, Hyun Ah Lee, Hee Sam Na, Chul Jong Jung, Gyung Yun Bek, and Jin Chung
목차
IntroductionMaterials and Methods
1. Bacterial culture
2. Preparation and treatment of CC extract and othernatural extracts
3. Screening experiments of herbal extracts against S.mutans and S. sobrinus
4. Measurement of growth of S. mutans and S. sobrinus
5. Viable cell count
6. Measurement of biofilm formation
7. Glucan synthesis test
Results
1. Screening of natural extract with antibacterialactivity
2. Effect of CC extract on the growth of S. mutansand S. sobrinus
3. Effect of CC extract on biofilm formation
4. Effect of CC extract on glucan synthesis
Discussion
Acknowledgements
Conflicts of Interest
References
영어 초록
Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1–2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract’s inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus . Therefore, C. chinensis might be a potential candidate for controlling dental caries.참고 자료
없음"International Journal of Oral Biology"의 다른 논문
Role of microglial activation on neuronal excitability ..7페이지
Antitumor effects of octyl gallate on hypopharyngeal ca..7페이지
Induction of anti-aquaporin 5 autoantibodies by molecul..7페이지
High-salt and glucose diet attenuates alveolar bone rec..7페이지
Increased SOX2 expression in three-dimensional sphere c..7페이지
Alteration of cellular events in tooth development by c..7페이지
Green tea polyphenol (–)-epigallocatechin-3-gallate pre..11페이지
L-ascorbic acid induces apoptosis in human laryngeal ep..10페이지
High concentration of calcium represses osteoblast diff..7페이지
Anticancer effects of D-pinitol in human oral squamous ..10페이지