Porphyromonas gingivalis lipopolysaccharide stimulates vascular smooth muscle cell migration through signal transducer and activator of transcription 3-mediated matrix metalloproteinase-9 expression
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서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 44권 / 1호
ㆍ저자명 : Yeon Kim, Joo-Yeon Park, Hyun-Joo Park, Mi-Kyoung Kim, Yong-Il Kim, Soo-Kyung Bae, Hyung Joon Kim, Moon-Kyoung Bae
목차
Introduction
Materials and Methods
1. 실험재료
2. 혈관평활근세포배양
3. RNA 분리와 역전사 중합효소연쇄반응(reverse transcription-polymerase chain reaction, RT-PCR)
4. 실시간 중합효소연쇄반응(real-time quantitative reverse transcription-PCR, real-time qRT-PCR )
5. Western immunoblot analysis
6. 혈관평활근세포의 이동성 분석(scratch wound migration assay)
7. 통계처리
Results
1. P. gingivalis LPS가 혈관평활근세포의 MMP-9 발현에 미치는 영향
2. P. gingivalis LPS가 유도한 MMP-9의 발현에 JAK2/STAT3 신호전달기전의 관련성
3. JAK2/STAT3 저해제가 P. gingivalis LPS가 유도하는 혈관평활근세포의 이동능에 미치는 영향
Discussion
References
영어 초록
Periodontal diseases have been associated with the development of cardiovascular diseases. Accumulating evidences have indicated that Porphyromonas gingivalis , a major periodontopathic pathogen, plays a critical role in the pathogenesis of atherosclerosis. In the present study, we demonstrated that P. gingivalis lipopolysaccharide (LPS) increases the mRNA and protein expression of matrix metalloproteinase-9 (MMP-9) in rat vascular smooth muscle cells. We showed that the MMP-9 expression induced by P. gingivalis LPS is mediated by the activation of signal transducer and activator of transcription 3 (STAT3) in vascular smooth muscle cells. Furthermore, the inhibition of STAT3 activity reduced P. gingivalis LPS-induced migration of vascular smooth muscle cells. Overall, our findings indicate that P. gingivalis LPS stimulates the migration of vascular smooth muscle cells via STAT3-mediated MMP-9 expression.
참고 자료
없음
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