Regulation of Inflammatory Response in Periodontal Ligament Cells by Transglutaminase 2

서지정보

ㆍ발행기관 : 대한구강생물학회 ㆍ수록지정보 : International Journal of Oral Biology / 42권 / 4호
ㆍ저자명 : Sun Young Lee, Cheol Hun Jang, Je-Hwang Ryu

목차

서 론
재료 및 방법
Periodontal ligament (PDL) 세포의 배양 및 처리
RNA isolation, Reverse transcription-polymerasechain reaction (RT-PCR) 및 quantitative real time-PCR (qRT-PCR)
면역형광염색 (Immunofluorescence)
자료 분석 및 통계처리
결 과
염증이 유발된 치주조직에서 TGM2의 발현 양상
TGM2가 과발현된 PDL세포에서 염증매개인자들의발현 변화
TGM2의 기능억제 시 PDL세포 내 염증매개인자들의발현 변화
고 찰
감사의 글
Conflict of interest
References

영어 초록

Transglutaminase2 (TGM2) is a multi-functional calcium dependent enzyme that affects angiogenesis, apoptosis, differentiation, attachment, and changes in the extracellular matrix. However, its function in periodontal tissue has not yet been studied. The aim of this study was to investigate the association of the TGM2 expression and the modulation of inflammatory mediators in inflamed periodontal ligament (PDL) cells induced by pro-inflammatory cytokines such as Interleukin-1β and the Tumor necrosis factor-α. The expression of TGM2 was increased in the inflamed periodontal tissue and PDL cells. Over-expressed TGM2 in the PDL cells increased expression of MMP1, MMP3, IL-6, CXCL8, and PTGS2. Conversely, inhibition of TGM2 activity using LDN27219, a TGM2 inhibitor, resulted in decreased expression of MMP1, MMP3, IL-6, and CXCL8. The mRNA expression was confirmed by RT-PCR and quantified by qRT-PCR. Protein levels were also confirmed by immunofluoroscence staining. These results suggest that TGM2 plays an important role in the regulation of inflammatory mediators which exacerbate tissue damage in inflamed periodontal tissue.

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