Immortalization of bovine primary epithelial cells to isolate foot-and-mouth disease virus
(주)코리아스칼라
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- 2023.04.05
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- 2014.03
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서지정보
ㆍ발행기관 : 한국예방수의학회(구 한국수의공중보건학회)
ㆍ수록지정보 : 예방수의학회지 / 38권 / 1호
ㆍ저자명 : Kyong-Leek Jeon, Kyu-Kye Hwang
영어 초록
A cell line of bovine origin was immortalized to isolate foot-and-mouth disease virus (FMDV). The immortalization was performed by infection of bovine primary epithelial cells with a recombinant retrovirus that overexpressed the human telomerase (hTERT), after primary culture of fetal bovine kidney tissue and removal of fibroblasts. After cloning the immor- talized cell line into single cells, the cloned cell lines were named JNUBK-1, JNUBK-2, JNUBK-3 and JNUBK-4, according to their characteristics. To confirm the epithelial phenotype of the cell lines JNUBK-3 and JNUBK-4, which showed stable proliferation capability over 35 generations after immortalization, the expression of cytokeratin and fibronectin was measured. Finally, the FMDV titer in the JNUBK-3 and JNUBK-4 cell lines was measured and was 800∼2,000 times higher than that of the currently used cell line IRBS-2. In conclusion, more sensitive isolation and production of FMDV became possible through the use of the immortalized JNUBK-3 and JNUBK-4 cell lines.
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