In Situ 방법을 이용한 단백질분해효소 처리 사료단백질의 반추위내 분해 특성에 관한 연구

서지정보

ㆍ발행기관 : 강원대학교 동물자원공동연구소 ㆍ수록지정보 : 동물자원연구 / 22권 / 2호
ㆍ저자명 : 임동현, 기광석, 이현준, 김상범, 권응기, 박중국

목차

ABSTRACT Ι. 서론 Ⅱ. 재료 및 방법 1. 시험사료 2. 사료단백질의 효소처리 3. 공시축 및 사양관리 4. Nylon bag 시험 5. 건물과 단백질의 분해율 6. 조사항목 및 분석방법 7. 통계분석 Ⅲ. 결과 및 고찰 Ⅳ. 요약 Ⅴ. 인용문헌

영어 초록

This study was conducted to investigate the effects on in situ ruminal degradation of feed protein sources (soybean meal, P-SBM; dried distillers grain with solubles, P- DDGS; wheat bran, P-WB) treated with protease as compared with conventional feed protein sources (soybean meal, SBM; dried distillers grain with solubles, DDGS; wheat bran, WB). There was no significant change in the chemical composition and amino acid profiles of enzyme treatment protein sources compared with the non-treated groups (p<0.05). But for treatment groups, the solid content and total amino acids were reduced by increasing the moisture content due to proteolytic conditions. On the entire incubation time in situ ruminal degradation rate of dry matter appeared higher in treatment groups compared to control groups (p<0.05), and that of the treatment groups suspended during 48 hours were in the order of P-SBM (97.70%), P-WB (74.26%) and P- DDGS (72.39%). In particular, DM degradation rate of enzyme treated DDGS significantly increased to 43.62%, 45.99%, 55.97%, 69.87% and 72.39%, respectively, incubated during 3, 6, 12, 24 and 48 hours in rumen (p<0.05). Also protein degradation rate of P-WB and P-SBM significantly decreased compared to their respective non-treated sources; however, by contrast, in DDGS it was increased. For P-SBM, protein degradation rate significantly decreased at 6 and 12 hours, and for P-DDGS it was increased at 3 and 6 hours of suspension times in rumen (p<0.05). In particular, protein degradation rate of enzyme treated group suspended for 48 hours were in the order of P-SBM (91.81%), P-WB (86.36%) and P-DDGS (58.87%). This result suggests that protease treatment of feed protein sources might be utilized to increase the bypass ratio into post-rumen for wheat bran, soybean meal, and to improve the utilization of feed protein.

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