Signal Transduction of Equine Follicle-Stimulating Hormone Receptor (eFSHR) by rec-eelFSHβ/α, Natural Porcine FSH, and Natural Human FSH
(주)코리아스칼라
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- 2023.04.05
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- 2018.03
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 42권 / 1호
ㆍ저자명 : Munkhzaya Byambaragchaa, Dae-Jung Kim, Myung-Hwa Kang, Kwan-Sik Min
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Materials
Construction of rec-eelFSH
Production and quantification of rec-eelFSHβ/α proteininCHO suspension cells
Transient transfection of eFSHR in CHO-K1 cells andstable transfection of eFSHR in PathHunter CHO-K1 EAParentalcells
Evaluation of cAMP level by homogenous time-resolvedfoster resonance energy transfer (HTRF)
Data analysis
RESULTS AND DISCUSSION
REFERENCES
영어 초록
In this study, we analyzed signal transduction by equine follicle-stimulating hormone receptor (eFSHR) on stimulation with recombinant eelFSHβ/α (rec-eelFSHβ/α), natural porcine FSH (pFSH), and natural human FSH (hFSH). cAMP stimulation in CHO-K1 cells expressing eFSHR was determined upon exposure to different doses (0-1450 ng/mL) of these hormones. The EC50 value of rec-eelFSHβ/α was 53.35 ng/mL. The Rmax values of rec-eelFSHβ/α and pFSH were 28.12 and 2.88 ng/mL, respectively. The activity of rec-eelFSHβ/α was much higher than that of natural pFSH. However, signal transduction in CHO PathHunter Parental cells expressing eFSHR was not enhanced by stimulation with natural hFSH. Thus, rec-eelFSHβ/α was completely active in cells expressing eFSHR. However, natural hFSH did not invoke a signal response in cells expressing eFSHR. Particularly, natural pFSH was weakly active in the same cells. These results showed that eelFSHβ/α has potent activity in cells expressing eFSHR. Thus, rec-eelFSHβ/α may efficiently bind to eFSHR, where as natural hFSH does not bind to eFSHR.
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