병풀(Centella asiatica) 착즙액과 추출물의 Asiaticoside 분석법 검증 및 항산화 활성
(주)코리아스칼라
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- 2023.05.22
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- 2023.04
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서지정보
ㆍ발행기관 : 한국식품영양학회
ㆍ수록지정보 : 한국식품영양학회지 / 36권 / 2호
ㆍ저자명 : 김연숙, 신현영, 하은지, 구자평, 정세빈, 김가을에, 정미연, 유광원
목차
Abstract
서 론
재료 및 방법
1. 시료 제조
2. 표준용액 및 시료 조제
3. HPLC 분석조건
4. 분석법 검증
5. 항산화 활성
6. 통계처리
결과 및 고찰
1. 재배환경에 따른 병풀 착즙액과 추출물의 유효성분 함량 분석
2. 분석법 검증
3. 항산화 활성
요약 및 결론
감사의 글
References
영어 초록
Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 μg/mL and LOQ was 15.12 μg/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.
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