Three Potyviruses Detected in the Bulbs of Narcissus

서지정보

ㆍ발행기관 : 한국화훼학회 ㆍ수록지정보 : 화훼연구 / 31권 / 3호
ㆍ저자명 : Eun Song Heo, Sun Hee Choi

목차

Introduction
Materials and Methods
Bulb sources of Narcissus
Extraction of total RNAs from the bulb tissues
Reverse transcription (RT)
Polymerase chain reaction (PCR) using universalprimers for potyvirus
Sequencing of PCR products amplified usingpoty-universal primers
Design of primers for specific virus detectionand PCR
Sequences generated from Narcissus isolates ofCyEVA, NYSLV, and NYSV, and phylogenetic construction
Results
Screening and identification of potyviruses inbulb tissues
Application of virus-specific primers for diagnosis
Identification of virus isolates obtained fromNarcissus spp. based on phylogeny
Discussion
Acknowledgment

영어 초록

Narcissus (Narcissus spp.) bulbs were used to investigate contagious viral retention in the dormant stage. The amplified polymerase chain reaction (PCR) bands were screened using universal potyvirus primers and sequenced to identify specific viruses. To identify individual viruses, primers for specific viruses were designed, and reverse transcription (RT)-PCR was performed. The PCR fragments were sequenced and compared by phylogenetic analysis. Three potyviruses, cyrtanthus elatus virus A (CyEVA), narcissus yellow stripe virus (NYSV), and narcissus late season yellows virus (NLSYV) were singly or doubly detected in 18 samples from six cultivars of the 38 samples representing eight cultivars that were tested. Based on RT-PCR using specific primers, CyEVA was detected in the split corona daffodil cv. Orangery. NLSYV and NYSV frequently occurred as co-infections with each other in the double daffodil cv. Tahiti, and the N. cyclamineus daffodil cv. Tete-a-tete and cv. Tete Boucle. Results has shown that at least three potyviruses are present in imported Narcissus bulbs.

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