P1 Transduction -Deletion of recA gene from E. coli chromosome
- 최초 등록일
- 2009.07.10
- 최종 저작일
- 2009.05
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한컴오피스
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소개글
P1 Transduction. recA gene을 deletion 시킨 내용에 관한 결과 레포트입니다
목차
1. Subject
2. Object
3. Introduction
4. Material & Method
5. Result
6. Discussion
7.Reference
본문내용
1. Subject: P1 Transduction
-Deletion of recA gene from E. coli chromosome
2. Object: P1 Transduction을 이해하고, E. coli chromosome에서 recA gene의 역할과 recA gene을 deletion 시키는 방법을 알아보자.
3. Introduction
(1) Bacteriophage
-Capsids or icosahedral heads
-Phage genome containing at least one copy of each of the phage genes
-landing module
(2) P1 phage
-Infects gram-negative bacteria
-Contains linear DNA in the head
-Adsorption of P1 tail fibers to the receptor is Ca+2-dependent.
-It forms a circle by recombination between terminally repeated sequences at its ends after infection
-The DNA then replicates as a circle and forms concatemers from which the DNA is packaged by a headful mechanism
-linear 형태로 들어와 linear form으로 남아있을수 있음.
-DNA가 circular form으로 존재 → 안정적이다.
(3)cre-lox system
-Site-specific한 recombination system
-DNA의 particular site에서 recombination을 진전시킴.
참고 자료
Michael T. Madigan 외/ BROCK BIOLOGY OF MICROORGANISMS 10th ed./ 2003/ Prentice Hall/ p. 258-260, 278-286, 542-545.
미생물학, 최호형, 아카데미서적, 2004, p.145~150
http://74.125.155.132/search?q=cache:b2iESFi5yZsJ:biotech.korea.ac.kr/lab/jsshin/class/200901KyoDaeWon/Chapter18.ppt+Lysogenic+cycle&cd=12&hl=ko&ct=clnk&gl=kr&lr=lang_ko
