RNA extract agents 종류와 역할

소개글

RNA 추출을 위해 protocol에 따르면 여러 agents 들이 사용되는데 이러한 agensts들의 종류와 그 역할을 기술

목차

1. Yeast cell lysis buffer
2. Phenol
3. RLT buffer
4. Ethanol

본문내용

□ Yeast cell lysis buffer

○ AE-buffer

- EDTA ( ethylenediaminetetraacetic acid )




EDTA is widely used for scavenging metal ions such as Ca2+ and Fe3+: In biochemistry and molecular biology, ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins
EDTA는 세포벽의 integrity를 유지하는 데 필수적인 calcium ion을 제거하여 세포벽이 군데군데 무너지게 합니다.

- sodium acetate




As the conjugate base of a weak acid, a solution of sodium acetate and acetic acid can act as a buffer to keep a relatively constant pH. This is useful especially in biochemical applications where reactions are pH dependent.

○ β-Mercaptoethanol (2-Mercaptoethanol)




2-Mercaptoethanol is used in some RNA isolation procedures to eliminate ribonuclease released during cell lysis. Numerous disulfide bonds make ribonucleases very stable enzymes, so 2-mercaptoethanol is used to reduce these disulfide bonds and irreversibly denature the proteins. This prevents them from digesting the RNA during its extraction procedure

참고 자료

없음