단백질 분석에 대한 실험과 원리

소개글

단백질 분석에 대한 실험과 원리

목차

1. ionic Properties of Amino acids and Proteins
2. Protein Structure
3. Protein Purification
4. Protein structure determination

본문내용

Electrostatic attractions that help to stabilize the three-dimensional structure of the protein molecule.
The relative numbers of positive and negative groups in a protein molecule influence aspects of its physical behavior, such as solubility and electrophoretic mobility.
PI is the pH at which the protein molecule possesses an equal number of positive and negative groups formed.

2. Protein Structure
Proteins are condensing, loss of a molecule of water and the formation of a peptide bond.

N- terminal amino acid is taken as the beginning of the chain and the C-terminal amino acid the end of the chain.
Peptides contain fewer than 50 AA residues and proteins
more than 50.
Ex1) Insulin (peptide hormone) - 53 AA residues
Ex2) Ribonuclease (small protein) - 103 AA residues

참고 자료

Principles and Techniques of Practical Biochemistry