분화 배지 및 초세포(CHO cell line) 무혈청 및 무단백 배지

소개글

Mesenchmal stem cell 분화(differentation medium) 배지
osteogenic,odontogenic differentiation medium
adipogenic differentiation medium
Cho cell 용 protein free medium (무단백배지) information 보유(010-5711-8372)

목차

Mesenchmal stem cell 분화(differentation medium) 배지
osteogenic,odontogenic differentiation medium
adipogenic differentiation medium
Cho cell 용 protein free medium (무단백배지) information 보유(010-5711-8372)

본문내용

Reagent stock solution concentrarion volume of Reagent Needed(mL) Final concentration
KH 0 0 0
α Final volume 404.5 Final volume 0
-MEM 0 75 15% 0
FBS 50x 5 180 mM 0
Glutamate 200 mM 5 2 mM 0
L-Ascorbic acid phosphate 1x 5 10000 U/ml 0
P/S antibiotics 40 ng/ml 0.5 10 μM 0
0.01 μM 10 mM 5 0.1 mM 0
Dexamethasone 0 500 0 0 0
2 0 0 0
α Final volume 398.25 Final volume
-MEM 0 75 15%
FBS 50x 5 180 mM
Glutamate 200 mM 5 2 mM
L-Ascorbic acid phosphate 1x 5 10000 U/ml
100 U/ml 1.8 mM 5 50 mM
Isobutylmethylxanthine 50 mM 0.5 4 mg/ml
Hydrocortisone 0.5 mM 5 0.5 μM
Indomethacin 6 mM 1.25 60 μM
Dexamethasone 0 500 0
PO 0 0 0
Insulin Final volume 484.45 Final volume
Neurobasal A bFGF 10 Final volume
B27 supplement 10 μg/ml 0.05 40 μg/ml
EGF 200 μg/ml 0.5 20 ng/ml
L-Ascorbic acid phosphate 1x 5 10000 U/ml
Dexamethasone 0 500 0
μ 0 0 0
Reagent stock solution concentrarion volume of Reagent Needed(mL) Final concentration
4 0 0 0
α Final volume 413 Final volume 0
-MEM 0 50 10% 0
A. Preparation of odontogenic differentiation medium 0 2 5mM 0
L-Ascorbic acid phosphate 1x 5 10000 U/ml 0
P/S antibiotics B. preparation of adipogenic differentiation medium 5 hate 0
A. Preparation of osteogenic differentiation medium 10 25 M 0
Dexamethasone 0 500 0 0 0

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