siRNA
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- 2010.06.21
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- 2009.05
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Small interfering RNA (siRNA) is a tool that is revolutionizing bioscience research.
목차
siRNA
siRNA in Bioscience Research
Limitations of Traditional Methodologies
Advantages of siRNA Over Alternatives
siRNA Products
siRNA Resources
1) siRNA (short interfering RNA)
본문내용
siRNA in Bioscience Research
Small interfering RNA (siRNA) is a tool that is revolutionizing bioscience research. The increased use of siRNA in peer reviewed publications clearly demonstrates that it is an incredibly powerful means to specifically knock-down a gene`s message, and subsequently the protein level of the targeted gene. In this manner, cellular-based assays can be conducted in the absence and presence of the targeted gene`s protein.
The value of such an experiment has long been known as researchers the world over have employed genetic knock-out technologies, dominant negatives and chemical inhibitors of protein activities to perform such experiments. The use of siRNA is replacing all three of these methodologies.
Limitations of Traditional Methodologies
Traditional methodologies for generating cellular "null" backgrounds have significant limitations. Genetic knock-out models, generated by interrupting a gene through the use of a targeting vector, are very labor intensive and costly to not only produce but also maintain.
As important as speed is for many researchers, cost is equally important. Experiments using siRNA can be performed at a fraction of the cost of generating genetic knock-out models and dominant negatives. In addition, siRNA mediated gene knock-down has been shown to be highly sequence specific.
This specificity is also characteristic of genetic knock-out models, but that comes at the cost of time and money when compared to using siRNA. In contrast, chemical inhibitors of protein activity and dominant negatives can be inexpensive and quick to use, but often are not specific for their intended target. In particular, chemical inhibitors provide a level of specificity for their target that is undesirable to many peer reviewed journals today. The use of siRNA in place of chemical inhibitors adds significantly to the specificity of the experiment making the resulting data far more credible without adding to its cost or time.
siRNA Products
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