Western blot 방법, 웨스턴 블랏 방법

소개글

Western blot 방법, 전기영동 실험 method

목차

1. Preparation of Running gel
2. Preparation of Stacking gel
3. Preparation of samples
4. Load the sample & Transfer
5. Blot for 2h at 15V
6. After blotting, dip for 10` in 5% formaldehyde and wash in DW for 10`
7. Wrap with Whatman paper and store at 4℃

본문내용

1. Preparation of Running gel (1.0mm / 2plates) : 물을 붓고 나서 선이 보이면 다 굳은 것

1-1. 1.5 M Tris-HCl, pH 8.8 (100 ml)
Tris base 18.165 g (FW 121.1)
adjust pH 8.8 with HCl

1-2. 1 M Tris-HCl, pH 6.8 (100 ml)
Tris base 12.1 g
adjust pH 6.8 with HCl

1-3. 10% ammonium persulfate (1 ml)
ammonium persulfate (APS) 0.1 g
: store at 4℃ in the dark. Fresh solutions should be prepared weekly (희석 후 일주일 정도 유효)

참고 자료

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