ELISA의 정의 및 종류

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ELISA의 정의 및 종류
준비하는데 많은 시간과 자료를 들였습니다.
후회하지 않으실겁니다.

목차

1. 면역학의 기초
2. About ELISA

본문내용

Ab = Antibody directed against antigen
AB = Species-specific antiserum, e.g, if Ab was raised in a mouse, anti-Ab is antimouse serum.
*E = Enzyme attached to a particular antibody ( Anti-Ab*E = Antispecies antibody linked to enzyme, e.g., antimouse)
I = Solid-phase to which reagent is attached passively
S = Substrate addition and color development
+ = Addition of reagents and incubation
Read = Measurement of color using spectrophotometer
W = Separation of bound and free reagents by washing
Direct ELISA의 원리
Direct method는 항원과 반응하는 항체에 바로 효소를 결합시켜 사용하는 방법이다.
Direct ELISA의 종류
1. Direct-Labeled Antibody
I-Ag + Ab*E + S -> Read
w w
2. Direct-Labeled Antigen
I-Ab + Ag*E + S -> Read
w w
Direct-Labeled Antibody
Stage i : Passive adsorption of antigen to plate
by incubation in defined buffer.
Stage ii : Wash.
Stage iii : Addition and incubation of enzyme-
labeled antibody.
Stage iv : Wash.
Stagge v : addition and incubation of color
development system.
Stage vi : Read.

III. Sandwich ELISA

IV. Competition ELISA

참고 자료

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