유전자적중법(gene targeting)의 3가지

소개글

유전자 적중법(gene targeting)에 관련한 최신 리뷰논문을 다수 참고하여 작성하였습니다.
(노벨상 관련 논문포함)

gene targeting의 3가지 type을 상세히 정리하였습니다.

목차

1. definition

2. contribution

3. gene targeting type

4. comparison of Knockout & Knock-in

5. Knock down (= RNAi) mechanism

6. The Review on Knockout

본문내용

1. Team Logo : ex) 뜨랑스

2. Definition :
Gene targeting is a genetic technique that uses homologous recombination to change an endogenous gene. Also called replacement strategy based on homologous recombination (source : wikipedia)

3. Contribution :

The gene targeting or knockout mouse made it possible to reverse the genetic approach to elucidate gene function compared to the classical forward genetics.
Thus, it is "reverse genetics" that allows us to conduct the "gene-driven mutagenesis" in the mosue

gene targeting 혹은 knockout mouse는 유전자돌연변이 유발 생쥐를 인위적으로 만들어,
교배와 표현형을 통해 생물학적 기능을 밝혀낸다.

(source :
Next-generation gene targeting in the mouse for functional genomics
Yoichi Gondo*, Ryutaro Fukumura, Takuya Murata & Shigeru Makino
Mutagenesis and Genomics Team, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan)

4. Type - Gene Targeting
I) Knock out : homologous recombination 으로 endogenous gene 변형
(특정 유전자 발현 제거) cf 1) flanking region 에 대해 알아야 할 것..!!
cf 2) 2007 Novel Prize
II) Knock in : homologous recombination 으로 endogenous gene 변형
(특정 유전자의 mutation이 도입)

참고 자료

http://www.nobelprize.org/
RNA interference,2006/2007 Novel Prize
Next-generation gene targeting in the mouse for functional genomics
Yoichi Gondo*, Ryutaro Fukumura, Takuya Murata & Shigeru Makino
Mutagenesis and Genomics Team, RIKEN BioResource Center, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan
n engl j med 357;24 www.nejm.org december 13, 2007